Micro Organisms Inactivated by Ozone

Most micro organisms (bacteria, protozoan, virus, and associated toxins) can be inactivated by the use of ozone. Below are some of the organisms inactivated by ozone. There are also some links for articles on ozone treatment of specific organisms at the bottom of the page.

Organism (Ozone Susceptibility)

Botrytis cinerea (3.8 mg/l for 2 minutes)
Bacteriophage f2 (99.99% destruction at 0.41 mg/l for 10-seconds in water)
Bacillus subtilis (90% reduction at 0.10-PPM for 33 minutes)
B. cereus (spores) (99% destruction after 5-min at 2.3 mg/l in water)
Bacillus cereus 99% destruction after 5-min at 0.12 mg/l in water
Bacillus Anthracis (Ozone susceptible)
Bacillus Bacteria (Destroyed by 0.2 m/l within 30 seconds)
Botulism (see link below)
Aspergillus Niger (Destroyed by 1.5 to 2 mg/l)
Klebs-Loffler Bacillus (Destroyed by 1.5 to 2 mg/l)
Influenza Virus (Destroyed 0.4 to 0.5 mg/l)
Herpes Virus (Destroyed in less than 30 seconds with 0.1 to 0.8 mg/l)
Hepatitis A Virus (99.5% reduction at 0.25 mg/l for 2-seconds)
GDVII Virus (Destroyed in less than 30 seconds with 0.1 to 0.8 mg/l)
Fusarium oxysporum f.sp. Melonogea (99.99 % destruction at 1.1 mg/l for 20 minutes)
Fusarium oxysporum f.sp. lycopersici (Destroyed by 1.1 mg/l for 10 minutes)
Enterovirus Virus (Destroyed in less than 30 seconds with 0.1 to 0.8 mg/l)
Endamoebic Cysts Bacteria (Ozone susceptibility)
Encephalomyocarditis Virus (Destroyed in less than 30 seconds with 0.1 to 0.8 mg/l)
E-coli (in wastewater) (99.9% destruction at 2.2 mg/l for 19 minutes)
E-coli (in clean water) (99.99% destruction at 0.25 mg/l for 1.6 minutes)
Escherichia Coli Bacteria (from feces) (Destroyed by 0.2 mg/l within 30 seconds in air)
Enteric virus (95% destruction at 4.1 mg/l for 29 minutes in raw wastewater)
Echo Virus 29 (1 minute at 1 mg/l of ozone, 99.999% killed)
Eberth Bacillus (Typhus abdomanalis) (Destroyed by 1.5 to 2 mg/l)
Diphtheria Pathogen (Destroyed by 1.5 to 2 mg/l)
Coxsackie Virus B5 (99.99% destruction at 0.4 mg/l for 2.5-minutes in sludge effluent)
Coxsackie Virus A9 (95% destruction at 0.035 mg/l for 10-seconds in water)
Clostridium Botulinum Spores. (Destroyed 0.4 to 0.5 mg/l)
Clostridium Bacteria (Ozone susceptibility)
Cladosporium (90% reduction at 0.10-PPM for 12.1 minutes)
Clavibacter michiganense 99.99% destruction at 1.1 mg/l for 5 minutes
Listeria (Ozone susceptibility, see link below)
Virbrio Cholera Bacteria Very susceptible
Vesicular Virus Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l
Verticillium dahliae 99.99 % destruction at 1.1 mg/l for 20 minutes
Streptococcus Bacteria Destroyed by 0.2 mg/l within 30 seconds
Stomatitis Virus Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l
Staphylococci Destroyed by 1.5 to 2.0 mg/l
Staph. epidermidis 90% reduction at 0.1-ppm for 1.7 min
Schistosoma Bacteria (High ozone susceptibility)
Salmonella typhimurium (99.99% destruction at 0.25 mg/l for 1.67 minutes in water)
Salmonella Bacteria (High ozone susceptibility, see link below)
Rhabdovirus virus (Destroyed in less than 30 seconds with 0.1 to 0.8 mg/l)
Pseudomonas Bacteria (High ozone susceptibility)
Proteus Bacteria (High ozone susceptibility)
Poliovirus type 1 (99.5% destruction at 0.25 mg/l for 1.6 minutes in water)
Poliomyelitis Virus (99.99% kill with 0.3 to 0.4 mg/l in 3-4 minutes)
Phytophthora parasitica (Destroyed 3.8 mg/l for 2 minutes)
Penicillium Bacteria (Ozone susceptibility)
Mycobacterium foruitum (90% destruction at 0.25 mg/l for 1.6 minutes in water)
Mycobacterium avium (99.9% with a CT value of 0.17 in water)
Mucor piriformis (Destroyed 3.8 mg/l for 2 minutes)
Luminescent Basidiomycetes (Destroyed in 10 minutes at 100-PPM)
Legionella pneumophila (99.99% destruction at 0.32 mg/l for 20 minutes in distilled water, see link below)

Below are some articles and references covering this topic:

Botulism Fact Sheet

Efficacy of Ozone for Inactivation of Legionella

Efficacy of Ozone for Inactivation of Various Food Borne Pathogens

Microbiological Aspects of Ozone Applications in Food

Ozone Inactivation of Listeria

Ozone in Food Safety

Ozone Inactivation of Salmonella